form
lyophilized powder
storage temp.
−20°C
Application
Suitable for use as a capillary zone electrophoresis calibration standard in uncoated capillaries. Using capillary zone electrophoresis, peptides migrate in free solution through a coated or uncoated fused silica capillary with an applied electric field. Proteins are separated by their charge-to-mass ratio. Narrow diameter capillaries (less than 100 μm) allow heat to dissipate efficiently so that high electric fields can be applied to the capillary, leading to high separation efficiencies of peptides. Using peptide standards for determining performance of the capillary is recommended before applying unknown samples. Sigma′s peptide standards have been selected to produce nine well-defined peaks using peptide separation buffer (P2188).
Preparation Note
Reconstitute with 50 μl of peptide separation buffer and 450 μl of deionized water.
Other Notes
Contains approx. 25 μg of each of nine peptides: Bradykinin, Bradykinin fragment 1-5, Substance P, [Arg8]-vasopressin, Luteinizing hormone releasing hormone, Bombesin, Leucine enkephalin, Methionine enkephalin, Oxytocin.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
Regulatory Information
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Zone electrophoresis in open tubular glass capillaries.
Jorgenson, J. and Lukacs, K.D.
Analytical Chemistry, 53, 1298-1298 (1981)
Capillary electrophoresis analysis of recombinant proteins.
G Teshima et al.
Methods in enzymology, 271, 264-293 (1996-01-01)
Kate J Treharne et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 24(5-6), 347-360 (2009-11-17)
Deletion of phenylalanine-508 (DeltaF508) from the first nucleotide-binding domain (NBD1) in the wild-type cystic fibrosis (CF) transmembrane-conductance regulator (wtCFTR) causes CF. However, the mechanistic relationship between DeltaF508-CFTR and the diversity of CF disease is unexplained. The surface location of F508
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