P2714
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder
Synonym(s):
Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor
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About This Item
Product Name
Protease Inhibitor Cocktail powder, for general use, lyophilized powder
biological source
bovine lung
Quality Level
form
lyophilized powder
solubility
water: soluble
storage temp.
−20°C
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General description
Application
- in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
- in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
- in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
- in 0.1 M NaCl solution to wash and collect skin secretions of frog
Biochem/physiol Actions
Preparation Note
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Other Notes
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
Disclaimer
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Choose from one of the most recent versions:
Certificates of Analysis (COA)
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How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?
One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.
How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use. Aliquots can be stored at -20 C for 1 month.
How much of Product P2714, Protease Inhibitor Cocktail, should I use with serum?
Dilute the contents of the vial in 10 mL of water, yielding a 10X initial concentration . Then dilute the stock 1:10 in the serum (1 part of inhibitor solution to 10 parts of serum). This would be a 1:100 dilution (containing the concentrations of each inhibitor as listed on the data sheet) in the serum. This is true for most cell lysates, serum, and culture media.
Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?
Yes, because it is water based.
Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?
No because it contains EDTA.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors S8820 and P2714?
S8820 is the tablet version of P2714.
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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