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Merck
CN

P2732

Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse

clone 6H11, tissue culture supernatant

Synonym(s):

Anti-BCDS2, Anti-CAS, Anti-CTNND, Anti-P120CAS, Anti-P120CTN, Anti-p120, Anti-p120(CAS), Anti-p120(CTN)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02097107

Key Documents

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Product Name

Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse, clone 6H11, tissue culture supernatant

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using cultured Madin Darby canine kidney (MDCK) epithelial cells
western blot: 1:10,000 using whole cell extract of cultured chicken fibroblasts

biological source

mouse

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

6H11, monoclonal

contains

15 mM sodium azide

species reactivity

bovine, canine, chicken, mouse, rat, human

isotype

IgG1

UniProt accession no.

O60716

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... CTNND1(1500)
mouse ... Ctnnd1(12388)
rat ... Ctnnd1(311163)

Application

Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA),
  • immunocytochemistry
  • immunoprecipitation

Biochem/physiol Actions

Reacts specifically with the N-terminal region of p120ctn and recognizes isoforms 1 and 2, but not isoforms 3 or 4. In immunoblotting, the antibody recognizes a 120 kDa band, and an additional very weak doublet at 200 kDa.
The 120 kD catenin-related protein p120ctn (also known as catenin p120ctn, p120cas and pp120 src substrate), was originally described as a prominent substrate for the sarcoma (Src) oncoprotein and for a variety of receptor tyrosine kinases (RTKs) including those for epidermal growth factor (EGF), platelet derived growth factor (PDGF), and colony stimulating factor-1 (CSF1). Like β-catenin and plakoglobin, it binds directly to the cytoplasmic domain of epithelial (E)-cadherin via the ARM domain and co-precipitates in multiprotein complexes containing E-cadherin or other cadherins such as neural (N)- or placental (P)- cadherins, depending on the cell type. However, unlike β-catenin and plakoglobin, p120ctn does not appear to bind α-catenin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-p120ctn (Catenin-related) (mouse IgG1 isotype) is derived from the 6H11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an A/J mouse immunized with a recombinant N-terminal fragment of murine p120ctn. Catenin δ-1 (p120ctn) contains a central armadillo repeat domain (ARM domain) and is structurally similar to β-catenin and plakoglobin. At least six alternatively spliced p120ctn isoforms are generated and differentially expressed in different cell types.

Immunogen

recombinant N-terminal fragment of murine p120ctn.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
108M4850V
22180540
010K4857

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J Wu et al.
Hybridoma, 17(2), 175-183 (1998-06-17)
This report describes the generation and characterization of a panel of monoclonal antibodies (MAb) to the catenin p120ctn. p120ctn (formerly p120cas) is a cadherin-binding protein with structural similarity to the classical catenins beta-catenin and plakoglobin. It was originally identified as
p120catenin alteration in cancer and its role in tumour invasion
Peglion F and Etienne-Manneville S
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1629), 20130015-20130015 (2013)
p120 Catenin Associates with Microtubules INVERSE RELATIONSHIP BETWEEN MICROTUBULE BINDING AND RHO GTPase REGULATION
Franz CM and Ridley AJ
The Journal of Biological Chemistry, 279(8), 6588-6594 (2004)
Plakophilins: multifunctional scaffolds for adhesion and signaling
Bass-Zubek AE, et al.
Current Opinion in Cell Biology, 21(5), 708-716 (2009)
Selective uncoupling of p120ctn from E-cadherin disrupts strong adhesion
Thoreson MA, et al.
The Journal of cell biology, 148(1), 189-202 (2000)
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