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Merck
CN

P2986

Pullulanase from Bacillus acidopullulyticus

aqueous solution, ≥400 units/mL

Synonym(s):

Promozyme 400L, Pullulan 6-glucano-hydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
232-983-9
MDL number:
EC Number:
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form

aqueous solution

specific activity

≥400 units/mL

density

1.25 g/mL at 25 °C (lit.)

storage temp.

2-8°C

General description

Heat-stable debranching enzyme obtained from a selected strain of Bacillus acidopullulyticus, and belongs to the group of debranching enzymes known as pullulanases.

Other Notes

One unit is defined as the amount of enzyme which hydrolyzes pullulan, liberating reducing carbohydrate with a reducing power equivalent to 1.0 μmole glucose per minute at pH 5.0 and 40 °C.

Legal Information

A product of Novozymes Corp.
Promozyme is a trademark of Novozymes Corp.

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Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Johanne Mørch Jensen et al.
Protein expression and purification, 79(2), 217-222 (2011-05-05)
The limit dextrinase inhibitor (LDI) from barley seeds acts specifically on limit dextrinase (LD), an endogenous starch debranching enzyme. LDI is a 14 kDa hydrophobic protein containing four disulfide bonds and one unpaired thiol group previously found to be either
Adam Waśko et al.
Preparative biochemistry & biotechnology, 41(3), 252-261 (2011-06-11)
This paper describes a simple and efficient method of isolation of a plullulanase type I from amylolytic lactic acid bacteria (ALAB). Extracellular pullulanase type I was purified from a cell-free culture supernatant of Lactococcus lactis IBB 500 by using ammonium
Bo Yu et al.
Molecules (Basel, Switzerland), 16(4), 3010-3017 (2011-04-09)
The effects of cyclodextrins and derivatives on the activity and structure of pullulanase were investigated in this study. Our results showed that cyclodextrins and derivatives decreased the activity of pullulanase. This decrease was attributed to the interaction between the hydrophobic
Haixia Li et al.
Plant cell reports, 30(9), 1641-1659 (2011-05-11)
High temperature has adverse effects on rice yield and quality. The different influences of night high temperature (NHT) and day high temperature (DHT) on rice quality and seed protein accumulation profiles during grain filling in indica rice '9311' were studied
Grzegorz Kłosowski et al.
Journal of bioscience and bioengineering, 109(5), 466-471 (2010-03-30)
The aim of the research was to assess the possibility of the fermentation productivity rising through the increase in corn mashes extract from 16-17 to 20-21 degrees Balling, yet keeping a 3-day fermentation period. The second goal was to obtain

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