P3397
Phosphoglucomutase from rabbit muscle
ammonium sulfate suspension, ≥100 units/mg protein
Synonym(s):
PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
Quality Segment
description
ammonium sulfate suspension, >=100 units/mg protein
form
ammonium sulfate suspension
specific activity
≥100 units/mg protein
storage condition
(Tightly closed)
technique(s)
activity assay: suitable
color
white to light yellow
foreign activity
lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%
shipped in
wet ice
storage temp.
2-8°C
General description
Research area: Cell Signaling
Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.
Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.
Application
Phosphoglycomutase has been used:
- to study glycogenesis and phosphoglycomutase deficiency in humans
- in phosphoglucomutase assays to study metabolic regulation
- for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
- in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
- in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP+)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)
Biochem/physiol Actions
Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4, pH 6.0 containing 0.01% EDTA
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.
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Storage Class
12 - Non Combustible Liquids
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