P4300
Pectinase from Rhizopus sp.
suitable for plant cell culture, BioReagent, crude powder
Synonym(s):
Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
product line
BioReagent
form
crude powder
specific activity
400-800 units/g solid
technique(s)
cell culture | plant: suitable
storage temp.
−20°C
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General description
Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Application
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Katsuichi Saito et al.
Microbiological research, 159(1), 83-86 (2004-05-27)
The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000
Shigenobu Yoshida et al.
Mycological research, 108(Pt 12), 1407-1414 (2005-03-11)
The polygalacturonase (PG)-encoding gene (rpg1) of Rhizopus oryzae, the causal pathogen of rhizopus rot of mulberry, was cloned and sequenced. PGs were partially purified from incubation mixture of 2% pectin medium and their N-terminal amino acid sequences were determined by
K Saito et al.
Journal of industrial microbiology & biotechnology, 30(7), 440-444 (2003-07-05)
Rhizopus oryzae strain NBRC 4707 produced lactic acid and ethanol more efficiently than strain NRRL 395 in potato pulp, an agricultural by-product of the starch industry. The two strains developed comparable activities of xylanase, cellulase, alpha-amylase, and glucoamylase, while the
Jeffrey A Mertens et al.
Fungal genetics and biology : FG & B, 45(12), 1616-1624 (2008-10-22)
A search of the recently sequenced Rhizopus oryzae strain 99-880 genome database uncovered 18 putative polygalacturonase genes with two genes being identical and only one with similarity to a previously reported R. oryzae polygalacturonase gene. The 17 different genes share
Isabelle Benoit et al.
BMC genomics, 13, 321-321 (2012-07-21)
Pectins are diverse and very complex biomolecules and their structure depends on the plant species and tissue. It was previously shown that derivatives of pectic polymers and oligosaccharides from pectins have positive effects on human health. To obtain specific pectic
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