P4300
Pectinase from Rhizopus sp.
suitable for plant cell culture, BioReagent, crude powder
Synonym(s):
Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
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About This Item
CAS Number:
UNSPSC Code:
12352200
EC Number:
232-885-6
MDL number:
product line
BioReagent
form
crude powder
specific activity
400-800 units/g solid
technique(s)
cell culture | plant: suitable
storage temp.
−20°C
General description
Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Application
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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