P4377
Phosphatase, Alkaline from Escherichia coli
ammonium sulfate suspension, ~10 units/mg protein (modified Warburg-Christian)
Synonym(s):
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-631-4
MDL number:
form
ammonium sulfate suspension
specific activity
~10 units/mg protein (modified Warburg-Christian)
storage temp.
2-8°C
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Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4377 has been used for cell digestion during measurement of photoreactivating enzyme
Biochem/physiol Actions
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Physical form
Suspension in 2.5 M (NH4)2SO4 solution
Preparation Note
Salt fractionated
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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B M Sutherland et al.
Journal of bacteriology, 138(2), 333-338 (1979-05-01)
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