P4404
Polyguanylic acid potassium salt
≥95% (TLC), enzyme from (synthesis), lyophilized powder
Synonym(s):
Poly(G) potassium salt
Product Name
Polyguanylic acid potassium salt, lyophilized powder
biological source
enzyme from (synthesis)
Quality Level
Assay
≥95% (TLC)
form
lyophilized powder
quality
≤5% free nucleotides
solubility
water: 19.60-20.40 mg/mL, clear to slightly hazy, colorless to light yellow
storage temp.
−20°C
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General description
Polyguanylic acid takes up a four-stranded helical structure. It is synthetically produced in Thermus thermophilus from guanosine diphosphate (GDP) and its synthesis is catalyzed by the enzyme polynucleotide phosphorylase.
Application
Polyguanylic acid (PolyG) is used to study the effects of ionizing radiation on formation and stability of G-quadruplex structures and as a target molecule for physicochemical studies of electronic excitation (vibrational spectra) of guanosine.
Polyguanylic acid potassium salt has been used:
- as a ligand for surface neuropilin-1 (NRP1) for internalization studies
- for intercalation studies with trisubstituted and disubstituted triazole-linked phenyl derivatives CL41, CL42 and CL2r50 using voltammetry method
- as a synthetic polynucleotide for agarose gel electrophoresis analysis
Biochem/physiol Actions
Polyguanylic acid acts as a ligand for scavenger receptor and reduces the surface expression neuropilin-1 in endothelial cells.
Preparation Note
Prepared from GDP using polynucleotide phosphorylase
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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A study of the pH dependence of electronically excited guanosine compounds by picosecond time-resolved infrared spectroscopy.
McGovern DA, Doorley GW, et al.
Photochemistry and Photobiology, 8, 542-548 (2008)
Picosecond infrared probing of the vibrational spectra of transients formed upon UV excitation of stacked G-tetrad structures.
McGovern DA, Quinn S, Doorley GW, et al.
Chemical Communications (Cambridge, England), 28, 5158-5160 (2007)
A Dora R Pontinha et al.
Bioelectrochemistry (Amsterdam, Netherlands), 101, 97-105 (2014-09-10)
The redox mechanism of two trisubstituted triazole-linked phenyl derivatives (CL41 and CL42) and a disubstituted triazole-linked phenyl derivative (CL2r50) were studied using cyclic, differential pulse and square wave voltammetry at a glassy carbon electrode. The CL41, CL42 and CL2r50 oxidation
D Grygoryev et al.
Radiation research, 173(1), 110-118 (2010-01-01)
The ability of guanine-rich sequences to form quadruplex structures in telomeres for example is important in a number of biological processes such as aging, carcinogenesis and gene regulation. Ionizing radiation can cause damage to guanine moieties that can affect the
Zhong-yuan Kan et al.
Nucleic acids research, 35(11), 3646-3653 (2007-05-10)
Chromosomes in vertebrates are protected at both ends by telomere DNA composed of tandem (TTAGGG)n repeats. DNA replication produces a blunt-ended leading strand telomere and a lagging strand telomere carrying a single-stranded G-rich overhang at its end. The G-rich strand
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