P4530
Protamine-Agarose
saline suspension
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About This Item
MDL number:
UNSPSC Code:
41106500
form
saline suspension
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~50 μg/mL binding capacity (DNA)
storage temp.
2-8°C
Application
Protamine-agarose is used in protein chromatography, affinity chromatography and specialty resins. Protamine-agarose has been used to determine that Pichia pastoris (methylotrophic yeast) provides a convenient heterologous system for the production of recombinant subunits of human type 2A protein phosphatase (PP2A). Protamine-agarose has also been used to purify and characterize a novel protamine kinase in HL60 cells as well as to study myocardial infarction.
Physical form
Suspension in 0.5 M NaCl containing 0.02% thimerosal
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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G M Walton et al.
Analytical biochemistry, 161(2), 425-437 (1987-03-01)
An efficient high yield three-step purification procedure for protein kinase C consisting of ion exchange, hydrophobic interaction, and substrate affinity chromatographies is described. Protein which appears homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis contains amino acid sequences, predicted from cDNA
M Chedin et al.
Carcinogenesis, 18(8), 1463-1472 (1997-08-01)
Two different protein tyrosine kinases were detected in the cytosolic fraction of different human tumor tissues. After partial purification, the two enzymes, which were highly active in breast tumor tissues, were characterized. One of them, soluble tyrosine kinase-1 (STK-1), represents
Bruce M Brown et al.
Biochemistry, 41(46), 13526-13538 (2002-11-13)
In steps of protein purification of bovine retinal protein phosphatase 2A (PP2A), phosducin dephosphorylation activity peaks coelute with a PP2A enzyme complex, shown by peptide sequence analysis to contain a B' subunit, B56 epsilon. Other PP2A complexes with a slightly
V B Lokeshwar et al.
The Journal of biological chemistry, 264(32), 19318-19326 (1989-11-15)
Treatment of Swiss mouse 3T3 cells and human epidermoid carcinoma A431 cells with protamine at 37 degrees C increased the 125I-epidermal growth factor (EGF) binding activity at 4 degrees C. The effect of protamine on the increase of 125I-EGF binding
M Gschwendt et al.
FEBS letters, 307(2), 151-155 (1992-07-28)
Murine epidermis contains PKC zeta and eta as evidenced by the application of specific antisera. PKC zeta predominates in the cytosol and PKC eta in the particulate fraction. PKC zeta is shown to be present also in other murine tissues
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