P5420
Pullulanase from Klebsiella pneumoniae
ammonium sulfate suspension, ≥5 units/mg protein (biuret)
Synonym(s):
Amylopectin 6-gluconohydrolase, Limit dextrinase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-983-9
MDL number:
form
ammonium sulfate suspension
specific activity
≥5 units/mg protein (biuret)
storage temp.
2-8°C
Application
The enzyme from Sigma has been used to estimate the pullulan content of the ethanol precipitates obtained from fermented agro-industrial wastes. It has also been used to the evaluate the branching of the maltodextrin fractions obtained from potato starch-derived maltodextrins (Paselli MD6) using ethanol precipitation methods.
Pullulanase is a glycolytic enzyme from Klebsiella pneumoniae that is used to hydrolyze pullulan, an α-1,6-linked homopolymer of maltotriose. Product P5420 has been used to degrade carbohydrate from white beans Phaseolus vulgaris L. in healthy humans and to study pullulan biosynthesis.
Biochem/physiol Actions
Pullulanase hydrolyzes α-1,6 linkages of starch. Apart from pullulan, the enzyme also hydrolyzes α-1,6 linkages of amylopectin and glycogen. Maltose is the smallest sugar that it can release from an α-(1,6)-linkages.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 6.2
Preparation Note
Highly purified by a modification of ion exchange chromatography.
Other Notes
One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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M A Finkelman et al.
Applied and environmental microbiology, 43(2), 483-485 (1982-02-01)
A simple radiometric microassay for extracellular polysaccharide elaboration by yeast-like cells of Aureobasidium pullulans was developed, based upon a procedure originally described by Catley (FEBS Lett. 20:174-176, 1972). Incorporation of [C]glucose into pullulan was linear with respect to time and
Pullulan content of the ethanol precipitate from fermented agro-industrial wastes.
Israilides CJ, et al.
Applied Microbiology and Biotechnology, 49(5), 613-617 (1998)
Fractionation of maltodextrins by ethanol
Defloor I, et al.
Journal of Chromatography A, 803 (1-2), 103-109 (1998)
L Noah et al.
The Journal of nutrition, 128(6), 977-985 (1998-06-18)
Resistant starch (RS) is thought to be present in large amounts in legume seeds; however, it has never been quantified in healthy humans. RS from cooked (atmospheric pressure) white beans was quantified in humans and pigs, and characterized to explain
Edward K Gilding et al.
Nature communications, 4, 1483-1483 (2013-02-14)
The production of adequate agricultural outputs to support the growing human population places great demands on agriculture, especially in light of ever-greater restrictions on input resources. Sorghum is a drought-adapted cereal capable of reliable production where other cereals fail, and
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