P5656
Modified Lowry Kit for Protein Determination (Peterson′s Modification of Micro-Lowry Method)
usage
sufficient for ~200 assays
IVD
for in vitro diagnostic use
Application
Uses sodium dodecyl sulfate for dissolving lipoproteins; offers excellent recovery of membrane proteins.
Biochem/physiol Actions
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve
Features and Benefits
•Complete kit for protein assays
•Most widely cited protein assay
•Less protein to protein variation than other assays
•Contains reagents that can remove interfering substances
•Most widely cited protein assay
•Less protein to protein variation than other assays
•Contains reagents that can remove interfering substances
Kit Components Only
Product No.
Description
- DOC solution 20 mL
- Folin-Ciocalteu phenol reagent 1 bottle
- Lowry Reagent 5 bottle
- Protein standard 5 vial(s)
- TCA solution 20 mL
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Assay of proteins in the presence of interfering materials.
A Bensadoun et al.
Analytical biochemistry, 70(1), 241-250 (1976-01-01)
A simplification of the protein assay method of Lowry et al. which is more generally applicable.
G L Peterson
Analytical biochemistry, 83(2), 346-356 (1977-12-01)
Protein measurement with the Folin phenol reagent.
O H LOWRY et al.
The Journal of biological chemistry, 193(1), 265-275 (1951-11-01)
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