Anti-phospho-CaM Kinase II (CAMKII) (pThr³⁰⁵) antibody produced in rabbit

Intracellular Calcium (Ca²⁺) acts as major secondary messenger that mediates signalling induced by hormones and neurotransmitters. Cellular responses to calcium are modulated by a family of Ca²⁺/Calmodulin dependent protein kinases. The members of this family include CaMK I, CAMK II and CAMK IV. These kinases mediate rapid, highly specific and reversible post-translational modification involving ATP. CaMK II is abundantly expressed in mammalian brain, with highest expression in neurons of hippocampus and cerebral cortex, and plays critical role in synaptic transmission, memory, learning and behaviour. CaMK II family has four members, CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK IIα?and β?isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMK IIγ and δ isoforms are expressed in all tissues. CaMK II is autophosphorylated at α-Thr286 residue in response to changes in the concentration of intracellular calcium. This may prolong physiological responses to temporary changes in Ca²⁺ concentrations. CaMK II regulates synaptic signalling by phosphorylating glutamate receptors in the neurons. A distinct CaMK II has also been implicated in the process of smooth muscle contraction by phosphorylation of myosin light chain kinase. Autophosphorylation of Thr305 inhibits the association of CaM Kinase II with PSD
The antibody recognizes rat CaM Kinase II α (50 kDa) and β (60 kDa) phosphorylated at Thr 305.

synthetic phosphopeptide corresponding to amino acids from rat brain CaM Kinase II threonine 305.

Anti-phospho-CaM Kinase II (CAMKII) (pThr³⁰⁵) antibody may be used at a working dilution of 1:1000 for immunoblotting using rat brain lysates.

Neat rabbit antiserum

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