P6018
Penicillinase from Bacillus cereus
Type II, lyophilized powder
Synonym(s):
β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
type
Type II
form
lyophilized powder
packaging
vial of ≥2.5 units (using cephaloridine), vial of ≥300 units (using benzylpenicillin)
storage temp.
2-8°C
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Application
Used in the production of penicillin.
Biochem/physiol Actions
Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.
Physical form
Crude, lyophilized powder containing buffer and zinc salts, and stabilized with bovine serum albumin
Analysis Note
Typical vial content: approx. 10 mg total protein (biuret)
Other Notes
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Regulatory Information
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Hasan Nazik et al.
The Southeast Asian journal of tropical medicine and public health, 43(5), 1178-1185 (2013-02-26)
Bacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a
Despina Kotsanas et al.
The Medical journal of Australia, 198(5), 267-269 (2013-03-19)
Clinical utility of carbapenem antibiotics is under threat because of the emergence of acquired metallo-β-lactamase (MBL) genes. We describe an outbreak in an intensive care unit (ICU) possibly associated with contaminated sinks. Four clusters of gram-negative bacteria harbouring the MBL
Grace Yim et al.
Canadian journal of microbiology, 59(2), 110-116 (2013-03-07)
Microbial populations in wastewater treatment plants (WWTPs) are increasingly being recognized as environmental reservoirs of antibiotic resistance genes. PCR amplicons for plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS were recorded in samples from a WWTP in Vancouver, British Columbia.
Lena P Setchanova et al.
Annals of clinical microbiology and antimicrobials, 12, 6-6 (2013-03-28)
Pneumococcal and Haemophilus influenzae type b (Hib) vaccines were introduced in our national immunisation program in April 2010. The aims of this retrospective, laboratory-based study were to determine the serotypes and antibiotic resistance of Streptococcus pneumoniae and H. influenzae isolates
Josefina Pastrana-Carrasco et al.
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 64(6 Pt 1), 535-540 (2013-03-22)
To determine the frequency of the gene qacEdelta1 and characterize the resistance to biocides of extended-spectrum beta-lactamases producing enterobacteriaceae (ESBL-PE) obtained from clinical isolates causing nosocomial infections. In total 59 ESBL-PE causing nosocomial infections were included: Klebsiella pneumoniae (35) and
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