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Merck
CN

P6018

Sigma-Aldrich

Penicillinase from Bacillus cereus

Type II, lyophilized powder

Synonym(s):

β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
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type

Type II

form

lyophilized powder

packaging

vial of ≥2.5 units (using cephaloridine)
vial of ≥300 units (using benzylpenicillin)

storage temp.

2-8°C

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Application

Used in the production of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Physical form

Crude, lyophilized powder containing buffer and zinc salts, and stabilized with bovine serum albumin

Analysis Note

Typical vial content: approx. 10 mg total protein (biuret)

Other Notes

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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A A Alsultan et al.
Journal of medical microbiology, 62(Pt 6), 885-888 (2013-03-23)
Carbapenem-resistant Acinetobacter baumannii is becoming increasingly prevalent in patients with diabetes mellitus in the Middle East. We examined the relationship of these bacteria and their resistance mechanisms to the diabetic disease status of patients in Saudi Arabia. Susceptibilities of 271
Elena De Vecchi et al.
Journal of medical microbiology, 62(Pt 6), 859-863 (2013-03-12)
Urinary tract infections (UTIs) are a common cause of bacteraemia in the elderly and are associated with a high probability of hospitalization. Despite the impact of UTIs on health status and quality of life, a limited number of studies have
Josefina Pastrana-Carrasco et al.
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 64(6 Pt 1), 535-540 (2013-03-22)
To determine the frequency of the gene qacEdelta1 and characterize the resistance to biocides of extended-spectrum beta-lactamases producing enterobacteriaceae (ESBL-PE) obtained from clinical isolates causing nosocomial infections. In total 59 ESBL-PE causing nosocomial infections were included: Klebsiella pneumoniae (35) and
Lena P Setchanova et al.
Annals of clinical microbiology and antimicrobials, 12, 6-6 (2013-03-28)
Pneumococcal and Haemophilus influenzae type b (Hib) vaccines were introduced in our national immunisation program in April 2010. The aims of this retrospective, laboratory-based study were to determine the serotypes and antibiotic resistance of Streptococcus pneumoniae and H. influenzae isolates
Hasan Nazik et al.
The Southeast Asian journal of tropical medicine and public health, 43(5), 1178-1185 (2013-02-26)
Bacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a

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