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P6056

Endoproteinase Arg-C from mouse submaxillary gland

Name: Endoproteinase Arg-C from mouse submaxillary gland
Brand: SIGMA

suitable for protein sequencing, lyophilized powder

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About This Item

CAS Number:

82047-85-6

UNSPSC Code:

12352202

NACRES:

NA.56

EC Number:

279-893-6

MDL number:

MFCD01324999

EC Number:

3.4.21.35 (BRENDA, IUBMB)

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form

lyophilized powder

Quality Level

200

packaging

vial of 5 μg

suitability

suitable for protein sequencing

storage temp.

−20°C

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Application

Endoproteinase Arg-C from mouse submaxillary gland has been used to study the syncytium formation in MERS-CoV (middle East respiratory syndrome coronavirus)-infected Vero cells in the presence of exogenous proteases. It has been used for the digestion of Rpl23ab (ribosomal protein L23ab)-containing fraction for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.

Biochem/physiol Actions

Endoproteinase Arg-C is a serine endoprotease from mouse submaxillary gland which hydrolyzes peptide bonds at the carboxyl side of arginyl residues. The enzyme has been shown to cleave Lys-Lys and Lys-Arg bonds, and all Arg-X bonds may not be hydrolyzed.

Other Notes

One unit will hydrolyze 1.0 μmole of Nα-p-tosyl-L-arginine methyl ester per min at pH 8.0 at 25 °C.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Information

低风险生物材料

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Analysis of endoproteinase Arg C action on adrenocorticotrophic hormone by capillary electrophoresis and reversed-phase high-performance liquid chromatography.

Krueger RJ, et al.

Journal of Chromatography A, 543, 451-461 (1991)

Global changes in and characterization of specific sites of phosphorylation in mouse and human histone H1 Isoforms upon CDK inhibitor treatment using mass spectrometry.

Leesa J Deterding et al.

Journal of proteome research, 7(6), 2368-2379 (2008-04-18)

Global changes in the phosphorylation state of human H1 isoforms isolated from UL3 cells have been investigated using mass spectrometry. Relative changes in H1 phosphorylation between untreated cells and cells treated with dexamethasone or various CDK inhibitors were determined. The

Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I.

P I Bastiaens et al.

Proceedings of the National Academy of Sciences of the United States of America, 93(16), 8407-8412 (1996-08-06)

We have devised a microspectroscopic strategy for assessing the intracellular (re)distribution and the integrity of the primary structure of proteins involved in signal transduction. The purified proteins are fluorescent-labeled in vitro and reintroduced into the living cell. The localization and

Structural basis for the nucleic acid binding cooperativity of bacteriophage T4 gene 32 protein: the (Lys/Arg)3(Ser/Thr)2 (LAST) motif.

J R Casas-Finet et al.

Proceedings of the National Academy of Sciences of the United States of America, 89(3), 1050-1054 (1992-02-01)

To identify the functional residues of the N-terminal B region of bacteriophage T4 gene 32 protein involved in its cooperative binding to single-stranded nucleic acids, a process dependent on homotypic protein-protein interaction, we have studied the interaction of the protein

Proteomics in Functional Genomics: Protein Structure Analysis (2000)

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Protocols

Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

阿达木单抗（adalimumab）肽图谱 LC-MS/MS 分析工作流程

基于优化的 LC-MS/MS 工作流程，通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。

Global Trade Item Number

SKU	GTIN
P6056-1VL	04061838217776

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