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Merck
CN

P6135

Sigma-Aldrich

Phospholipase C from Bacillus cereus

Type III, lyophilized powder, 100-200 units/mg protein (using soybean L-α-phosphatidylcholine)

Synonym(s):

Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase

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About This Item

CAS Number:
9001-86-9
EC Number:
3.1.4.3 (BRENDA, IUBMB)
EC Number:
232-638-2
MDL number:
MFCD00131895
UNSPSC Code:
12352204

Key Documents

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type

Type III

form

lyophilized powder

specific activity

100-200 units/mg protein (using soybean L-α-phosphatidylcholine)

composition

Protein, ~10% Lowry

storage temp.

−20°C

Gene Information

Bacillus cereus ATCC 10987 ... plC(2749087)

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Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Physical form

Contains Tris buffer salts and ZnSO4

Other Notes

One unit will liberate 1.0 μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
043K4125
042K4097
039F4027
023H4044

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Shigeki Nakamura et al.
PLoS pathogens, 7(1), e1001247-e1001247 (2011-01-22)
Non-typeable Haemophilus influenzae (NTHi), a common commensal of the human pharynx, is also an opportunistic pathogen if it becomes established in the lower respiratory tract (LRT). In comparison to colonizing isolates from the upper airway, LRT isolates, especially those associated

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