P7286
Pyruvate Kinase from rabbit liver
Type XII, essentially salt-free, lyophilized powder, 10-20 units/mg protein
Synonym(s):
ATP: pyruvate 2-O-phosphotransferase, PK
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type
Type XII
form
essentially salt-free, lyophilized powder
specific activity
10-20 units/mg protein
foreign activity
lactic dehydrogenase, creatine phosphokinase and myokinase <0.01%
storage temp.
−20°C
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37°C in the presence of 1.0 mM of fructose 1,6-diphosphate. In the absence of added fructose 1,6-diphosphate which acts as an activator, considerably lower
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Daniel I Benjamin et al.
Cell metabolism, 16(5), 565-577 (2012-10-16)
Cancer cells possess fundamentally altered metabolism that provides a foundation to support tumorigenicity and malignancy. Our understanding of the biochemical underpinnings of cancer has benefited from the integrated utilization of large-scale profiling platforms (e.g., genomics, proteomics, and metabolomics), which, together
Weidong Zhou et al.
Cancer genomics & proteomics, 9(6), 373-381 (2012-11-20)
A variety of genomic and proteomic tools have been used to study cancer metabolism and metabolomics in order to understand how cancer cells survive in their environment. Throughout the past decade, mass spectrometry has been routinely used for large-scale protein
Robert D Christensen et al.
Blood cells, molecules & diseases, 50(2), 105-109 (2012-11-06)
We report a series of neonates who developed a total serum bilirubin (TSB) >20mg/dL during a recent ten-year period in a multihospital healthcare system. The incidence of a TSB >20mg/dL fell after instituting a pre-hospital discharge bilirubin screening program in
Nicolas Galazis et al.
European journal of endocrinology, 168(2), R33-R43 (2012-10-25)
Women with polycystic ovary syndrome (PCOS) are at increased risk of developing insulin resistance and type 2 diabetes mellitus (T2DM). In this study, we attempted to list the proteomic biomarkers of PCOS and T2DM that have been published in the
William J Cook et al.
PloS one, 7(10), e46875-e46875 (2012-10-12)
Pyruvate kinase plays a critical role in cellular metabolism of glucose by serving as a major regulator of glycolysis. This tetrameric enzyme is allosterically regulated by different effector molecules, mainly phosphosugars. In response to binding of effector molecules and substrates
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