P7397
Plasminogen from human plasma
lyophilized powder, ≥2 units/mg protein
Synonym(s):
Profibrinolysin
biological source
human plasma
form
lyophilized powder
specific activity
≥2 units/mg protein
mol wt
90 kDa
composition
, ~2%
concentration
~2% protein (Lowry)
suitability
not suitable for streptokinase determination by clot formation procedure
UniProt accession no.
foreign activity
plasmin <20% of base activity
storage temp.
−20°C
Gene Information
human ... PLG(5340)
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General description
Native-intact human plasminogen is a 791 amino acid glyco-protein with as many as 24 disulfide bonds. Plasminogen contains a single N-linked sialylated biantennary glycan. The two O-glycans possess a Gal β-1-3GalNAc core which are α-2-3 sialylated at the terminal Gal. An additional disialylated form has a second sialic acid residue with an α-2-6 linkage to GalNAc. Mono- and disialylated forms occur at a molar ratio of 80:20 in human plasminogen.
Biochem/physiol Actions
Plasminogen is the inactive precursor of the protease plasmin. Plasminogen is activated by the action of either tissue plasminogen activator (tPA), which primarily activates the fibrinolytic (thrombolytic) activity of plasmin, or urokinase plasminogen activator (uPA), which is associated with extracellular matrix remodeling and cell migration. Plasmin cleaves fibrin/fibrinogen and blood coagulation factors V/Va and VIII/VIIIa. It activates matrix metalloproteinases by cleaving the inactive proenzymes. It is also involved in the activation of some growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor β (TGF-β).
Physical form
Lyophilized powder containing NaCl, EDTA, lysine and Tris buffer
Preparation Note
Solutions should be stored in buffer containing 20 mM lysine at neutral pH. The protein should be as concentrated as possible. Frozen in aliquots at −20 °C, it should be stable for several weeks.
Analysis Note
ε-Aminocaproic acid free
Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.
Other Notes
One unit will produce a ΔA275 of 1.0 from α-casein in 20 min at pH 7.5 at 37 °C, when measuring perchloric acid soluble products in a volume of 5.0 mL. Activity determined after activation to plasmin with urokinase.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Oonagh Shannon et al.
Current topics in microbiology and immunology, 368, 189-205 (2012-12-12)
Haemostasis is maintained by a tightly regulated coagulation system that comprises platelets, procoagulant proteins, and anticoagulant proteins. During the local and systemic response to bacterial infection, the coagulation system becomes activated, and contributes to the pathophysiological response to infection. The
Z Rottenberger et al.
Journal of thrombosis and haemostasis : JTH, 11(3), 529-538 (2013-01-03)
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Liver-artery interactions via the plasminogen-CD36 axis in macrophage foam cell formation: new evidence for the role of remote organ crosstalk in atherosclerosis.
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To assess the concentration of adiponectin, soluble E-selectin, soluble thrombomodulin and tissue activator plasminogen antigen in postmenopausal women who received oral or transdermal hormone therapy. Case-control study. Polish university hospitals. Seventy-six healthy postmenopausal women. Forty-six women who received oral (n
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