P7498
PDK1 Active human
recombinant, expressed in E. coli, ≥75% (SDS-PAGE), aqueous glycerol solution
Synonym(s):
3-phosphoinositide-dependent protein kinase
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About This Item
MDL number:
UNSPSC Code:
12352200
recombinant
expressed in E. coli
Assay
≥75% (SDS-PAGE)
form
aqueous glycerol solution
specific activity
10 units/mg protein (Bradford)
mol wt
protein apparent mol wt ~59 kDa
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDPK1(5170)
Application
Kinase activity is measured as the molar amount of phosphate incorporated into PDKtide substrate peptide (KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC) per minute per mg protein at 30 °C using a final concentration of 50 microM [32P] ATP.
Biochem/physiol Actions
PDK1 plays a regulatory role in glucose and carbohydrate metabolism. It functions through the PI3K signaling cascade upstream of AKT1 as well as in other pathways involving proliferation, survival and cell migration.
Physical form
Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.
Other Notes
Human PDK1 is a 556-residue monomeric enzyme comprised of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases and a C-terminal pleckstrin homology domain.
One unit will incorporate one nanomole of phosphate into the PDK tide substrate (KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Rosa Ferriero et al.
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