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Merck
CN

P7786

Millipore

Protein A-Agarose from Staphylococcus aureus

aqueous suspension

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About This Item

MDL number:
UNSPSC Code:
41106500
NACRES:
NA.56
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form

aqueous suspension

matrix

Cross-linked 4% beaded agarose

matrix activation

p-nitrophenyl chloroformate

matrix attachment

amino

matrix spacer

1 atom

capacity

~10 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

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General description

Protein A is an immunoglobulin (Ig)-binding protein expressed in Staphylococcus aureus. It is used to purify large amounts of IgG and binds to the fraction of crystallization (Fc) part of the antibody at the CH2–CH3 interface. Protein A-agarose might be suitable for low-pressure antibody isolation. Protein A-agarose is used for affinity chromatography, antibody purification, and characterization, and protein A, G, and L-resins.

Application

Protein A-Agarose from Staphylococcus aureus has been used in immunoprecipitation. It has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Physical form

Suspension in 0.9% NaCl containing 0.1% sodium azide

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Deepak Bhardwaj et al.
Plant signaling & behavior, 6(2), 287-292 (2011-02-26)
We here report in Pisum sativum system a novel protein-protein interaction of β-subunit of heterotrimeric G-proteins (PsGβ) with a Mitogen activated protein kinase (PsMPK3) during cDNA library screening by yeast-two-hybrid assay. The transcript of these two genes also showed co-regulation
Mamta Behl et al.
Neurotoxicology, 31(5), 524-532 (2010-05-22)
The choroid plexus (CP), constituting the blood-cerebrospinal fluid barrier, has the capacity to remove beta-amyloid (Abeta) from the cerebrospinal fluid. Our previous work indicates that exposure to lead (Pb) results in Abeta accumulation in the CP by decreasing the expression
A C Melo et al.
Insect biochemistry and molecular biology, 30(7), 549-557 (2000-06-14)
The synthesis and secretion of vitellogenin by the ovary of Rhodnius prolixus was investigated. Using whole ovary or epithelial cells isolated from follicles of different sizes, it is shown that the follicle cells are a site of synthesis for this
Gloria P Larson et al.
Cell reports, 29(8), 2175-2183 (2019-11-21)
All viruses balance interactions between cellular machinery co-opted to support replication and host factors deployed to halt the infection. We use gene correlation analysis to perform an unbiased screen for host factors involved in influenza A virus (FLUAV) infection. Our
Lise Goltermann et al.
Protein engineering, design & selection : PEDS, 24(1-2), 123-129 (2010-11-05)
In bacteria, the 5' mRNA coding region plays an important role in determining translation output. Here, we report synthetic sequences that when placed in the 5'-mRNA coding region, leading to recombinant proteins containing short N-terminal extensions, virtually abolish, enhance or

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