P8023
Phospholipase D from Streptomyces chromofuscus
lyophilized powder, ≥2,000 units/mg solid
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
41106300
EC Number:
232-639-8
MDL number:
form
lyophilized powder
specific activity
≥2,000 units/mg solid
composition
Protein, ~50% Lowry
storage temp.
−20°C
Biochem/physiol Actions
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
One unit will liberate 1.0 μM of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 8.0 at 30°C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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N S Ryder et al.
Cellular signalling, 5(6), 787-794 (1993-11-01)
Phosphatidic acid (PA) induced a rapid dose-dependent increase in production of inositol phosphates in cultured adult human keratinocytes, peaking at 30 s. Natural and dioleoyl PA were equally effective, while other phospholipid classes had no effect. Lipid A was also
Viviana Trezza et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(43), 14899-14908 (2012-10-27)
The brain endocannabinoid system plays a crucial role in emotional processes. We have previously identified an important role for endocannabinoids in social play behavior, a highly rewarding form of social interaction in adolescent rats. Here, we tested the hypothesis that
Anna O Müller et al.
Chemistry and physics of lipids, 165(7), 787-793 (2012-10-13)
Cardiolipin (CL) and related diphosphatidyl lipids are hardly accessible because of the complexity of their chemical synthesis. In the present paper, the transphosphatidylation reaction catalyzed by phospholipase D (PLD) from Streptomyces sp. has been proven as an alternative enzyme-assisted strategy
Fumiyuki Sanematsu et al.
The Journal of biological chemistry, 288(12), 8092-8100 (2013-01-31)
Activation of receptor tyrosine kinases leads to the formation of two different types of plasma membrane structures: peripheral ruffles and dorsal ruffles. Although the formation of both ruffle types requires activation of the small GTPase Rac, the difference in kinetics
Wahida H Ali et al.
PloS one, 8(1), e55325-e55325 (2013-02-06)
Cell migration and phagocytosis ensue from extracellular-initiated signaling cascades that orchestrate dynamic reorganization of the actin cytoskeleton. The reorganization is mediated by effector proteins recruited to the site of activity by locally-generated lipid second messengers. Phosphatidic acid (PA), a membrane
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