P8685
Phospholipase A2 from Streptomyces violaceoruber
lyophilized powder, ≥10 units/mg solid
Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-637-7
MDL number:
biological source
Streptomyces sp. (S. violaceoruber)
form
lyophilized powder
specific activity
≥10 units/mg solid
storage temp.
−20°C
Application
Phospholipase A 2 is an enzyme used to hydrolyze phospholipids. It is used to study the release of arachidonic acid from various cell types such as neutrophils, gastric mucosal cells and kidney cells.
Biochem/physiol Actions
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
Phospholipase A 2 (PLA2) hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. It aggressively attacks phospholipids in membranes of intact cells. PLA 2specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. .
Physical form
Lyophilized powder containing mannitol and Tris buffer.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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J Marshall et al.
Journal of immunology (Baltimore, Md. : 1950), 164(4), 2084-2091 (2000-02-05)
The purpose of this study was to define the role of secretory phospholipase A2 (sPLA2), calcium-independent PLA2, and cytosolic PLA2 (cPLA2) in arachidonic acid (AA) release from fMLP-stimulated human neutrophils. While fMLP induced the release of extracellular sPLA2 activity and
Zhanglin Ni et al.
The Journal of biological chemistry, 281(24), 16245-16255 (2006-04-11)
Gastric epithelial cells liberate prostaglandin E(2) in response to cytokines as part of the process of healing of gastric lesions. Treatment of the rat gastric epithelial cell line RGM1 with transforming growth factor-alpha and interleukin-1beta leads to synergistic release of
Diversity of group types, regulation, and function of phospholipase A2.
E A Dennis
The Journal of biological chemistry, 269(18), 13057-13060 (1994-05-06)
Ming Chen et al.
Scientific reports, 7(1), 15931-15931 (2017-11-23)
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The
Willem van de Veen et al.
The Journal of allergy and clinical immunology, 131(4), 1204-1212 (2013-03-05)
IL-10-producing regulatory B cells suppress immune responses, and lack of these cells leads to exacerbated symptoms in mouse models of chronic inflammation, transplantation, and chronic infection. IgG4 is a blocking antibody isotype with anti-inflammatory potential that is induced in human
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