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Merck
CN

P8998

Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody produced in rat

clone 5H4, tissue culture supernatant, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
MDL number:
MFCD07785414

Key Documents

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biological source

rat

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

5H4, monoclonal

form

buffered aqueous solution

mol wt

antigen 65 kDa

species reactivity

Xenopus, human, mouse

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:5,000-1:10,000 using 293T cells transfected with PP2Aα

isotype

IgM

UniProt accession no.

P67775

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PPP2CA(5515)
mouse ... Ppp2ca(19052)

General description

PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates Ras-1 activation, localization of Sgo1 at centromeres, and chromosome segregation . This enzyme primarily comprises of three components; structural subunit A, regulatory subunit B and catalytic subunit C. The scaffolding subunit A has 2 isoforms (α and β) that have been implicated in tumor suppression
Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody is specific for human PP2Aα (approx. 65 kDa) and cross-reacts with Xenopus laevis and mouse PP2Aα. The antibody epitope resides within repeat 1 near the N-terminus of human PP2Aα.

Immunogen

Purified human PP2Aα subunit

Application

Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody is suitable for use in western blot at a dilution of 1:250 using protein extracts from human mucosal and esophageal tumor tissues . The product may also be used for immunoprecipitation, microarray and indirect ELISA.

Physical form

Supplied as a culture supernatant containing 15 mM sodium azide.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
065K4752

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E Kremmer et al.
Molecular and cellular biology, 17(3), 1692-1701 (1997-03-01)
Protein phosphatase 2A (PP2A) holoenzyme is composed of a catalytic subunit, C, and two regulatory subunits, A and B. The A subunit is rod shaped and consists of 15 nonidentical repeats. According to our previous model, the B subunit binds
Anna A Sablina et al.
Cell adhesion & migration, 1(3), 140-141 (2007-07-01)
The protein phosphatase 2A (PP2A) family of heterotrimeric serine-threonine phosphatases participates in human cell transformation. Each functional PP2A complex contains one structural A subunit (A alpha or A beta), and mutations of both are found to occur at low frequency
He-Ge Chen et al.
PloS one, 4(9), e7019-e7019 (2009-09-15)
Protein phosphatases-2A (PP-2A) is a major serine/threonine phosphatase and accounts for more than 50% serine/threonine phosphatase activity in eukaryotes. The holoenzyme of PP-2A consists of the scaffold A subunit, the catalytic C subunit and the regulatory B subunit. The scaffold
D Abraham et al.
The Journal of biological chemistry, 275(29), 22300-22304 (2000-05-10)
The Raf-1 kinase plays a key role in relaying proliferation signals elicited by mitogens or oncogenes. Raf-1 is regulated by complex and incompletely understood mechanisms including phosphorylation. A number of studies have indicated that phosphorylation of serines 259 and 621
Satoshi Ugi et al.
Molecular and cellular biology, 22(7), 2375-2387 (2002-03-09)
Protein phosphatase 2A (PP2A) is a multimeric serine/threonine phosphatase that carries out multiple functions. Although numerous observations suggest that PP2A plays a major role in downregulation of the mitogen-activated protein (MAP) kinase pathway, the precise mechanisms are unknown. To clarify
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