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Merck
CN

P9370

Monoclonal Anti-PRMT3 antibody produced in mouse

~2 mg/mL, clone PRMT3-367, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Protein Arginine Methyl Transferase 3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD05665444
Conjugate:
unconjugated
Clone:
PRMT3-367, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
3

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PRMT3-367, monoclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

mouse, rat, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using whole cell extracts of 293T cell line transfected with human PRMT3

isotype

IgG3

UniProt accession no.

O60678

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PRMT3(10196)

General description

Monoclonal Anti-PRMT3 (mouse IgG3 isotype) is derived from the PRMT3-367 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Protein arginine methyltransferase 3 (PRMT3), one of the type I arginine methyltransferases is expressed in heart, small intestine, kidney, ovary, testis, thyroid, and cortex with a cytoplasmic localization within the cells. It consists of two important domains in its N-terminal region, a C2H2 zinc-finger motif and a tyrosine-phosphorylation consensus sequence. This gene is located on human chromosome 11p15.1.

Immunogen

synthetic peptide corresponding to amino acids 501-514 of rat PRMT3 conjugated to KLH.

Application

Monoclonal Anti-PRMT3 antibody produced in mouse may be used in:
  • immunoblotting
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Does not cross-react with PRMT1, 2, 4, 5, or 6.
Protein arginine methyltransferase 3 (PRMT3) helps to catalyze the formation of asymmetric dimethylarginine. It plays a key role in ribosomal function and also in controlling protein synthesis. In apolipoprotein E (apoE) knockout mice, PRMT3 activity inhibition decreases hepatic steatosis. PRMT3 also serves as a specific cofactor for liver X receptor (LXR) transcription.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
026M4772V
093K4828

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Protein arginine methylation in estrogen signaling and estrogen-related cancers
Teyssier C, et al.
Trends in Endocrinology and Metabolism, 21(3), 181-189 (2010)
Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3)
Swiercz R, et al.
The Biochemical Journal, 386(1), 85-91 (2005)
Inhibition of PRMT3 activity reduces hepatic steatosis without altering atherosclerosis susceptibility in apoE knockout mice
Hoekstra M, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(6), 1402-1409 (2019)

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