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Merck
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P9529

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

lyophilized powder

Synonym(s):

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

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About This Item

CAS Number:
9040-59-9
UNSPSC Code:
12352202
EC Number:
232-920-5
MDL number:
MFCD00131864
EC Number:
3.1.4.17 (BRENDA, IUBMB)

Key Documents

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biological source

bovine brain

form

lyophilized powder

specific activity

1-5 units/mg protein (without added calmodulin)

mol wt

~60 kDa

composition

Protein, 4.0-6.0% Lowry

UniProt accession no.

P14100

foreign activity

2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%, 5′-ATPase ≤0.2%, inorganic pyrophosphatase ≤0.8%

storage temp.

−20°C

Gene Information

cow ... PDE1A(281969)

General description

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.

Application

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units

Physical form

Lyophilized powder containing Tris-HCl buffer salts and lactose

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

Analysis Note

Protein determined by Warburg-Christian

Other Notes

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
096K7415
089H7440
071H95205
071H95203
071H95201

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P D Calvert et al.
The Journal of general physiology, 111(1), 39-51 (1998-02-14)
Light adaptation in vertebrate photoreceptors is thought to be mediated through a number of biochemical feedback reactions that reduce the sensitivity of the photoreceptor and accelerate the kinetics of the photoresponse. Ca2+ plays a major role in this process by
M D Houslay
Seminars in cell & developmental biology, 9(2), 161-167 (1998-05-26)
Cyclic AMP has provided the paradigm for the second messenger concept. Recent evidence has identified a complex array of isoforms of the enzymes involved in generation, destroying and transmitting the effects of this second messenger. These proteins provide a sophisticated
Vibrio vulnificus Biotype 3 Multifunctional Autoprocessing RTX Toxin Is an Adenylate Cyclase Toxin Essential for Virulence in Mice
Kevin J. Ziolo
Infection (Munich) (2014)
Drummond, G.I. And Yamamoto, M. et al.
The Enzymes (1971)
Reciprocal regulation of calcium dependent and calcium independent cyclic AMP hydrolysis by protein phosphorylation
Kok-Long Ang Ferenc A. Antoni
Journal of Neurochemistry (2002)
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