P9620
Puromycin dihydrochloride
Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture
biological source
Streptomyces alboniger
Quality Level
type
for cell culture
Assay
>98% (HPLC)
form
solution
storage condition
(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )
concentration
10 mg/mL in H2O
technique(s)
cell culture | mammalian: suitable
color
colorless to yellow
solubility
H2O: soluble 10 mg/mL
antibiotic activity spectrum
Gram-positive bacteria
neoplastics
parasites
Mode of action
protein synthesis | interferes
shipped in
wet ice
storage temp.
−20°C
SMILES string
Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1
InChI
1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
InChI key
MKSVFGKWZLUTTO-FZFAUISWSA-N
Related Categories
General description
Puromycin′s structural similarity to the amino acid-bearing end of tRNA allows it to enter the ribosome during protein synthesis, bind to the nascent polypeptide chain, and halt chain elongation. Widely used in cell culture, puromycin serves as a selective agent for cells that have been transformed with a gene conferring resistance to puromycin.
Application
Biochem/physiol Actions
Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.
Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
Features and Benefits
- High-quality antibiotic suitable for multiple research applications
- Antibiotic and protein synthesis inhibitor
- Effective against prokaryotic and eukaryotic cells
- Inhibits protein synthesis by interfering with RNA function
- Commonly used as a selective agent in cell biology research
Preparation Note
Other Notes
comparable product
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
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