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PCG2004

Sigma-Aldrich

TruPAGE Precast Gels

4-20%, 10 x 10cm, 12-well

Synonym(s):
Precast Polyacrylamide Gels, Precast Gels for SDS-PAGE
NACRES:
NB.22

shelf life

24 mo. at 2‑8 °C (from the date of manufacture)

packaging

box of 10 ea

concentration

4-20% polyacrylamide

cassette W × L

10 cm × 10 cm

gel thickness

1 mm

well size × volume

12 wells × 35 μL

compatibility

for use with Life Technologies XCell SureLock® XCell I, XCell II Mini-Cell and Bolt® Mini Gel Tank
for use with Lonza Ltd PAGEr Minigel Chamber

storage temp.

2-8°C

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Application

TruPAGE Tris-MOPS SDS Express Running Buffer has been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Features and Benefits

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The precast polyacrylamide gels for SDS-PAGE come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.

TruPAGE Precast Gels Migration Chart
There is no tape to remove. There is also no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place.

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Bolt is a registered trademark of Life Technologies
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
PAGEr is a trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC
XCell I is a trademark of Life Technologies
XCell II is a trademark of Life Technologies
XCell SureLock is a registered trademark of Life Technologies

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Detection of phosphorylated Akt and MAPK in cell culture assays.
Molgaard S, et al.
MethodsX, 3, 386-398 (2016)
Pin-Chao Liao et al.
Cell reports, 32(2), 107902-107902 (2020-07-16)
The mitochondria-associated degradation pathway (MAD) mediates ubiquitination and degradation of mitochondrial outer membrane (MOM) proteins by the proteasome. We find that the MAD, but not other quality-control pathways including macroautophagy, mitophagy, or mitochondrial chaperones and proteases, is critical for yeast
Simon Molgaard et al.
MethodsX, 3, 386-398 (2016-06-09)
This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite
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Autologous chondrocyte implantation (ACI) has been used over the last two decades to treat focal cartilage lesions aiming to delay or prevent the onset of osteoarthritis; however, some patients do not respond adequately to the procedure. A number of biomarkers
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Nature, 513(7518), 436-439 (2014-07-22)
Fibroblast growth factor 1 (FGF1) is an autocrine/paracrine regulator whose binding to heparan sulphate proteoglycans effectively precludes its circulation. Although FGF1 is known as a mitogenic factor, FGF1 knockout mice develop insulin resistance when stressed by a high-fat diet, suggesting a

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