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PCG3009

Sigma-Aldrich

TruPAGE LDS Sample Buffer

4 ×

NACRES:
NB.22

concentration

4 ×

compatibility

for use with Sigma-Aldrich TruPAGE Precast Gels

storage temp.

2-8°C

General description

TruPAGE LDS Sample Buffer should ALWAYS be used to prepare protein samples for use with TruPAGE precast gels to achieve optimal band resolution and sharpness. The sample buffer is specifically formulated to complement the TruPAGE running buffer system and to deliver the optimal denaturing conditions without causing sample degradation. Effective protein denaturation occurs at 70 °C instead of the much higher temperature required with Laemmli buffer.

Application

TruPAGE LDS Sample Buffer-4× has been used in Western blotting.

Features and Benefits

TruPAGE LDS Sample Buffer is specially formulated to be used with TruPAGE Precast Gels to provide optimal sample loading conditions for gel electrophoresis. TruPAGE LDS Sample Buffer can also be supplemented with TruPAGE DTT Sample Reducer if reducing sample conditions are desired. Please refer to the gel migration chart to select the appropriate gel and running buffer combination for your experimental needs.

Legal Information

TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Blum D, et al.
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Quick and facile preparation of histone proteins from the green microalga Chlamydomonas reinhardtii and other photosynthetic organisms.
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Methods, 184, 102-111 (2020)
Minzi Deng et al.
Experimental cell research, 395(1), 112175-112175 (2020-07-18)
Autophagy is a basic catabolic response that eukaryotic cells use to degrade unnecessary or dysfunctional cellular components in an orderly and regulated manner. It plays important roles in maintaining cellular homeostasis, energy homeostasis, response to environmental stimuli, and the development
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Each approach used to synchronize cell cycle progression of human cell lines presents a unique set of challenges. Induction synchrony with agents that transiently block progression through key cell cycle stages are popular, but change stoichiometries of cell cycle regulators

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