PLA0201
Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified
Powered by Bethyl Laboratories, Inc.
Synonym(s):
14-3-3 sigma, Stratifin, YWHAS, epithelial cell marker protein 1
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Species reactivity:
human
Citations:
11
Technique(s):
immunohistochemistry: 1:2,000-1:10,000
immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000- 1:10,000
immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000- 1:10,000
Product Name
Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
immunohistochemistry: 1:2,000-1:10,000
immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000- 1:10,000
accession no.
NP_006133.1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SFN(2810)
Application
Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified has been used in the pull down assay for Rho associated coiled-coil containing protein kinase 1 (ROCK1) proteins and heat shock 70 kDa 4 protein (Hsp74) in HCT116 cancer cells.
Biochem/physiol Actions
Rabbit anti-14-3-3 or stratifin participates in several biological events including apoptosis and cell differentiation. It regulates the transcription matrix metallopeptidases (MMPs) in fibroblasts and may serve as therapeutic potential in wound healing. 14-3-3σ is less expressed and is hyper methylated in breast cancer cells. It plays a key role in linking signalling proteins and in the enhancing of protein kinase C functionality. A mutation in the stratifin gene is implicated in epilation phenotype in mice. It interacts with ubiquitin-specific protease 8 (USP8) and is highly expressed in human lung adenocarcinoma.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Rabbit anti-14-3-3 Sigma or stratifin is mapped to human chromosome 1p36.11. 14-3-3σ protein is expressed as seven isoforms and has molecular weight in the range of 25 to 30 kDa. It is an acidic homodimeric protein and harbors pleckstrin homology (PH) domain. 14-3-3σ is highly expressed in epidermis.
Immunogen
The epitope recognized by PLA0201 maps to a region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810).
Other Notes
14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.
Physical form
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
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A mutation in stratifin is responsible for the repeated epilation (Er) phenotype in mice
Herron BJ, et al.
Nature Genetics, 37(11), 1210-1210 (2005)
Stratifin, a keratinocyte specific 14-3-3 protein, harbors a pleckstrin homology (PH) domain and enhances protein kinase C activity
Dellambra E, et al.
Journal of Cell Science, 108(11), 3569-3579 (1995)
Hsp74/14-3-3sigma Complex Mediates Centrosome Amplification by High Glucose, Insulin, and Palmitic Acid
Lu YC, et al.
Proteomics, 1800197-1800197 (2019)
Stratifin regulates stabilization of receptor tyrosine kinases via interaction with ubiquitin-specific protease 8 in lung adenocarcinoma
Kim Y, et al.
Oncogene, 37(40), 5387-5387 (2018)
Mutation analysis of FANCD2, BRIP1/BACH1, LMO4 and SFN in familial breast cancer
Lewis AG, et al.
Breast Cancer Research, 7(6), R1005-R1005 (2005)
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