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PLA0303

Sigma-Aldrich

Goat anti-53BP1 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):
TP53, 53BP1, p202, TDRD30, p53-binding protein 1, p53BP1, tumor protein p53-binding protein, 1, tumor protein 53-binding protein
NACRES:
NA.41

Quality Level

biological source

goat

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human

concentration

1 mg/mL

application(s)

immunohistochemistry: 1:500-1:2,000
immunoprecipitation (IP): 2- 10 μg/mg

accession no.

NP_005648.1

shipped in

wet ice

storage temp.

2-8°C

Gene Information

goat ... 53BP1(7158)

General description

p53 binding protein 1 (53BP1), also known as, tumor protein p53 binding protein 1, is homologous to the DNA damage checkpoint protein Rad9p of Saccharomyces cerevisiae. In the human chromosome, 53BP1 is localized on 15q15.3.

Immunogen

The epitope recognized by PLA0303 maps to a region between residue 350 and 400 of human Tumor Protein p53 Binding Protein, 1 using the numbering given in entry NP_005648.1 (GeneID 7158).

Biochem/physiol Actions

p53 binding protein 1 (53BP1) is a transcriptional activator of tumour suppressor protein p53. 53BP1 senses the double strand DNA breaks induced by ionising radiations and chemicals and involved in the DNA repair pathway. 53BP1 is a downstream target of ataxia telangiectasia mutated (ATM) phosphorylation. 53BP1 binds to histone 3 lysine 79 methylated residues (H3K79) or dimethylated histone 4 lysine 20 (H4K20me2) via the tandem tudor domain, which enables its interaction with the double strand DNA breaks.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

53BP1 is a haploinsufficient tumor suppressor and protects cells from radiation response in glioma
Squatrito M, et al.
Cancer Research, canres-ca0045 (2012)
p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks
Schultz LB, et al.
The Journal of Cell Biology, 151(7), 1381-1390 (2000)
Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways
Rappold I, et al.
The Journal of Cell Biology, 153(3), 613-620 (2001)
Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks
Huyen Y, et al.
Nature, 432(7015), 406-411 (2004)
Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair
Botuyan MV, et al.
Cell, 127(7), 1361-1373 (2006)

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