PM0100
p38 MAPK ELISA
sufficient for 96 multiwell tests
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About This Item
UNSPSC Code:
12352200
usage
sufficient for 96 multiwell tests
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... MAPK14(1432)
General description
A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).
Application
Designed to detect and quantify the levels of native and recombinant p38 MAPK from cell lysates.
For research use only. Not for use in diagnostic procedures.
p38 MAPK ELISA specifically measures human, monkey, and mouse p38 MAPK.
p38 MAPK ELISA specifically measures human, monkey, and mouse p38 MAPK.
p38 MAP kinase (MAPK), also known as a CDC-2-related protein kinase or CSBP (cytokine suppressive anti-inflammatory drug binding protein) regulates many cellular processes including inflammation, cell differentiation, cell growth and death. p38 MAP kinase is the human homolog of the S. cerevisiae gene Hog1, which is a MAPK required for growth under high-osmolarity conditions. The kit is designed to detect and quantify the levels of phosphorylated and non-phosphorylated p38 MAPK from cell lysates. It detects native and recombinant p38 protein.
Features and Benefits
The assays are a sensitive, quantitative, and economical alternative to traditional immunoblotting or bioassays. They are fast (4 hours), easy to perform (precoated plates, room temperature incubations, no proprietary equipment required) and the results are reproducible from lot-to-lot.
Analysis Note
The sensitivity is <16 pg/mL of human p38 MAPK, which is 10x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.
Kit Components Only
Product No.
Description
- p38 MAPK Standard (lyophilized, human, full length recombinant p38 MAPK protein expressed in E. coli) 2 vial(s)
- Standard Diluent Buffer 1 x 25
- Anti-p38 MAPK-Coated 96 Well Plate 1 ea
- Anti-p38 MAPK Antibody 1 x 11
- Anti-Rabbit IgG-Horseradish Peroxidase (HRP) Concentrate (100x) 1 vial
- Wash Buffer Concentrate 25x 1 x 100
- Stabilized Chromogen, Tetramethylbenzidine (TMB) 1 x 25
See All (9)
Regulatory Information
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Instructions
Datasheet
Pouran Karimi et al.
Journal of cardiovascular and thoracic research, 5(2), 61-65 (2013-11-20)
Atherosclerosis is one of the inflammatory underlying disease associated by oxidative stress and thrombotic agents. This study aimed to evaluate the potential role of cupper oxidized low-density lipoprotein (OxLDL) and thrombin for inducing mitogen activated protein kinases (MAPKs) in platelets.
Nadereh Rashtchizadeh et al.
Journal of cardiovascular and thoracic research, 7(3), 107-112 (2015-10-03)
This study aimed to discover by which mechanism selenium (Se) suppresses stimulated platelets stimulation in oxidative stress underlying diseases. Human platelets pretreated with Se and stimulated by Cu(2+)-oxidized low density of lipoprotein (OxLDL) or thrombin before assessment of P-selectin and
J Han et al.
Biochimica et biophysica acta, 1265(2-3), 224-227 (1995-03-16)
Mitogen-activated protein (MAP) kinases are intracellular serine/threonine kinases activated by dual phosphorylation of adjacent threonine (T) and tyrosine (Y). A diverse number of extracellular signals induce activation of MAP kinases. Here we describe the cloning of a cDNA encoding human