PROT20
ProteoPrep® 20 Plasma Immunodepletion Kit
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About This Item
UNSPSC Code:
12352200
shipped in
wet ice
storage temp.
2-8°C
General description
Proteoprep 20 is an immunodepletion kit that removes 20 abundant interferring proteins from plasma or serum to allow analysis of less abundant proteins. Proteoprep 20 removes: albumin, IgG, transferrin, fibrinogen, IgA, α2- Marcroglobulin, IgM, α1- Antitrypsin, complement C3, haptoglobulin, apolipoprotein A1, A3 and B; α1- Acid Glycoprotein, ceruloplasmin, complement C4, C1q; IgD, prealbumin, and plasminogen.
Application
The ProteoPrep 20 Plasma Immunodepletion Kit specifically removes 20 of the most abundant proteins from human plasma or serum in preparation for further proteomics analysis. This depletion enables deeper penetration into the plasma proteome whether you use one- or two-dimensional electrophoresis, single or multidimensional chromatography or go straight to mass spectrometry. This is based on a unique conjugation process using small recombinant immunoaffinity ligands and conventional antibodies that permits high density conjugation on the support. The ligand selection ensures optimal specificity. Typical depletions remove 97-98% of the total protein mass in human plasma or serum.
Features and Benefits
• Increase your ability to visualize low abundance proteins and biomarkers – Removal of >99% of twenty of the most abundant proteins allows up to 50-fold increased protein loads, enhancing opportunities to visualize low abundance proteins
• Unique antibody affinity media – Novel high density antibody media displays higher specificity, reducing contamination and significantly improving depletion capacity compared to any currently available products
• Convenient spin-column format – Fast depletion and recovery accelerates throughput and validation making this the preferred choice for all proteomics labs
• Unique antibody affinity media – Novel high density antibody media displays higher specificity, reducing contamination and significantly improving depletion capacity compared to any currently available products
• Convenient spin-column format – Fast depletion and recovery accelerates throughput and validation making this the preferred choice for all proteomics labs
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
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Not applicable
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Not applicable
Regulatory Information
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Tara K Sigdel et al.
Frontiers in medicine, 4, 80-80 (2017-07-04)
Identification and use of non-invasive biomarkers for kidney transplantation monitoring is an unmet need. A total of 121 biobanked sera collected from 111 unique kidney transplant (KT) patients (children and adolescent) and 10 age-matched healthy normal controls were used to
Removal of albumin from multiple human serum samples.
K Rengarajan et al.
BioTechniques, 20(1), 30-32 (1996-01-01)
Tara K Sigdel et al.
Pediatric transplantation, 12(7), 737-747 (2008-09-04)
The desire for biomarkers for diagnosis and prognosis of diseases has never been greater. With the availability of genome data and an increased availability of proteome data, the discovery of biomarkers has become increasingly feasible. However, the task is daunting
Jon M Jacobs et al.
Journal of proteome research, 4(4), 1073-1085 (2005-08-09)
Candidate proteomic biomarker discovery from human plasma holds both incredible clinical potential as well as significant challenges. The dynamic range of proteins within plasma is known to exceed 10(10), and many potential biomarkers are likely present at lower protein abundances.
Tatiana Plavina et al.
Journal of proteome research, 6(2), 662-671 (2007-02-03)
We report on the development of a robust and relatively high-throughput method for in-depth proteomic analysis of human plasma suitable for biomarker discovery. The method consists of depletion of albumin and IgG and multi-lectin affinity chromatography (M-LAC), followed by nanoLC-MS/MS
Related Content
We have developed a novel, high-binding capacity, antibodybased resin for the depletion of twenty high abundance proteins in human plasma. The technology is a significant improvement over existing kits that remove 2, 6, or 12 proteins.
Investigation of Non-Specific Protein:Protein Interactions Following Removal of Twenty High Abundance Proteins from Human Plasma
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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