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Merck
CN

PROTIA

Millipore

ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

Key Documents

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technique(s)

immunodepletion: suitable

Quality Level

200

storage temp.

2-8°C

Related Categories

General description

ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.
The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.

Application

ProteoPrep® Immunoaffinity Albumin & amp; IgG Depletion Kit has been used in:
  • umbilical cord serum proteomics
  • human plasma (HP) proteomics
  • proteomic profile-based screening of potential protein biomarkers in the urine of patients with nephrotic syndrome

Features and Benefits

  • High Capacity removes more interfering abundant proteins
  • High Specifity ensures you see an accurate and more complete proteome
  • Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Regulatory Information

低风险生物材料
常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000437776
0000437776
SLCL4825
SLCL3124
SLCH8965

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. My protein samples contain urea.  Can I still use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, for depletion?

    The presence of urea can reduce the depletion of albumin with PROTIA.  We would suggest a buffer exchange prior to depletion using either dialysis or 5 KDa NMWL cut-off spin filters.

  4. Can I use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, on samples besides serum?

    Besides human serum or plasma samples, we have not specifically tested other human biological fluids with this kit.  However, in principle, there is no reason not to try the kit with other fluid samples.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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