Quantum Fluorescence Kit for MESF Units of FITC

The Quantum Fluorescence Kits for MESF units of FITC use flow cytometry to create a fluorescence intensity standard which matches the spectral properties of the cell sample. MESF (Molecules of Equivalent Soluble Fluorochrome) units are a relative fluorescence intensity unit which compares a stained sample to the fluorescence intensity of a known solution of equivalent fluorochrome molecules. This is done in a fluorimeter where both the sample and the standard fluorochrome solutions are exposed to the same local environment. MESF units are fluorochrome specific. Four FITC reference standards of microbeads (approximately the size of lymphocytes) are calibrated against solutions of laser grade FITC in terms of MESF units per microbead standard. The range of MESF units of FITC is 10,000 to 500,000. An unstained microbead population serves as negative control by indicating the background noise level of the flow cytometer.
A QuickCal Disk is supplied with every kit, which contains serial numbered folders holding lot specific information (i.e., kit name, assigned calibration values, lot numbers, expiration date). The encrypted information contained in this diskette can be read with the QuickCal v2.1 executable program (sold separately, Stock No. QCAL-10) to generate calibration plots, calculate instrument performance parameters and determine the fluorescence intensity of samples in MESF units of FITC.