R0762
Sau3A I from Staphylococcus aureus
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
1000-5000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
Sau3AI is a DNA restriction enzyme that cuts DNA at the recognition sequence 5′-/GATC-3′ to generate DNA fragments containing 5′-cohesive termini.
Biochem/physiol Actions
Recognition sequence: 5′-/GATC-3′
Cutting results: A 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 20 minutes.
Cutting results: A 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 250 mM NaCl, 5 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: One unit cleaves approximately 98% of the substrate DNA.
Sau3A I activity is not blocked by dam methylation.
Sau3A I activity is not blocked by dam methylation.
Isoschizomers: MboI
Supplied with 10x Restriction Enzyme Buffer SA (B7531)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Jason P Breves et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 158(2), 194-200 (2010-11-09)
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J S Sussenbach et al.
Nucleic acids research, 3(11), 3193-3202 (1976-11-01)
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes
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C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Yu-Feng Huang et al.
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Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
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