R1026
Anti-phospho-c-Raf (pTyr340/341) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
clone
polyclonal
form
buffered aqueous glycerol solution
usage
sufficient for 10 blots
species reactivity
rat, human, mouse
technique(s)
western blot: 1:1,000 using HEK293 cells overexpressing c-Raf
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... RAF1(5894)
mouse ... Zhx2(387609)
General description
Raf-1 is a serine/threonine kinase that is activated by tyrosine kinases . Raf-1 inhibits Ras signaling and subsequently modulates cell proliferation and development . c-Raf expression has been linked to the pathogenesis of lung carcinoma . Anti-phospho-c-Raf pTyr340/341 antibody is specific for human c-Raf that is phosphorylated on tyrosines 340 and 341. Although the product has not been tested for cross-reactivity, the antibody is expected to recognize mouse and rat c-Raf (100% homologous).
Immunogen
synthetic phosphopeptide derived from a region of human Raf that contains tyrosines 340 and 341.
Application
Anti-phospho-c-Raf pTyr340/341 antibody is suitable for use in western blot (1:1,000 using HEK293 cells overexpressing c-Raf).
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free), 50% glycerol, and 0.05% sodium azide.
Regulatory Information
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J A Thorson et al.
Molecular and cellular biology, 18(9), 5229-5238 (1998-08-26)
By binding to serine-phosphorylated proteins, 14-3-3 proteins function as effectors of serine phosphorylation. The exact mechanism of their action is, however, still largely unknown. Here we demonstrate a requirement for 14-3-3 for Raf-1 kinase activity and phosphorylation. Expression of dominant
D K Morrison
Molecular reproduction and development, 42(4), 507-514 (1995-12-01)
Raf-1 is a key protein involved in the transmission of developmental and proliferative signals generated by receptor and nonreceptor tyrosine kinases. Biochemical and genetic studies have demonstrated that Raf-1 functions downstream of activated tyrosine kinases and Ras and upstream of
J R Fabian et al.
Molecular and cellular biology, 13(11), 7170-7179 (1993-11-01)
The serine/threonine kinase activity of the Raf-1 proto-oncogene product is stimulated by the activation of many tyrosine kinases, including growth factor receptors and pp60v-src. Recent studies of growth factor signal transduction pathways demonstrate that Raf-1 functions downstream of activated tyrosine
Rafael B Blasco et al.
Cancer cell, 19(5), 652-663 (2011-04-26)
We have investigated the role of individual members of the Raf/Mek/Erk cascade in the onset of K-Ras oncogene-driven non-small cell lung carcinoma (NSCLC). Ablation of Erk1 or Erk2 in K-Ras oncogene-expressing lung cells had no significant effect due to compensatory
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