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Merck
CN

R1145

RPMI-1640 Medium

Without ʟ-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonym(s):

RPMI Medium, RPMI-1640 Culture Medium, Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

Key Documents

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Product Name

RPMI-1640 Medium, 10 ×, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Preparation Note

Supplement with 0.3 g/L L-glutamine, 2.0 g/L sodium bicarbonate, 0.001 g/L folic acid at 1×.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
RNBL9922
RNBL5425
RNBK6529
RNBK0721
RNBJ1360

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