R1259
Afl III from Anabaena flos-aquae
buffered aqueous glycerol solution
form
buffered aqueous glycerol solution
concentration
5,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition Sequence: 5′-A/CPuPyGT-3′
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Physical form
Solution in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1mM EDTA, 200 ug/ml BSA, 0.15% Triton X-100, 50% Glycerol (v/v) , pH 8.0 at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
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P R Whitehead et al.
Journal of general microbiology, 131(4), 951-958 (1985-04-01)
Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be: (formula; see text) AflII and AflIII are new specificities and may be
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Amal M El-Shehaby et al.
Scandinavian journal of clinical and laboratory investigation, 73(1), 75-81 (2012-12-04)
Left ventricular hypertrophy (LVH) is a common manifestation of cardiovascular disease and has an important prognostic value in patients with end-stage renal disease (ESRD). Vitamin D receptor (VDR) has been intensively investigated, and one of these (BsmI) already has been
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
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