R1259
Afl III from Anabaena flos-aquae
buffered aqueous glycerol solution
form
buffered aqueous glycerol solution
concentration
5,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition Sequence: 5′-A/CPuPyGT-3′
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Ligation and recutting results: After 2-10-fold Afl III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and 90% recut.
Heat inactivation: Complete inactivation at 80 °C for 20 minutes. 50% activity retained after 20 min at 65 °C incubation.
Physical form
Solution in 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1mM EDTA, 200 ug/ml BSA, 0.15% Triton X-100, 50% Glycerol (v/v) , pH 8.0 at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
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P R Whitehead et al.
Journal of general microbiology, 131(4), 951-958 (1985-04-01)
Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be: (formula; see text) AflII and AflIII are new specificities and may be
Diana Schenkwein et al.
Nucleic acids research, 41(5), e61-e61 (2013-01-01)
Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-specific gene insertion at desired genomic loci.
Beata Podgórska et al.
Acta biochimica Polonica, 59(4), 669-672 (2012-11-07)
In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
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