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Merck
CN

R2507

Reactive Blue 4−Agarose

saline suspension

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About This Item

NACRES:
NA.56
PubChem Substance ID:
24899356
UNSPSC Code:
23151817
MDL number:
MFCD00001218

Key Documents

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InChI

1S/C23H14Cl2N6O8S2/c24-21-29-22(25)31-23(30-21)28-12-7-9(5-6-14(12)40(34,35)36)27-13-8-15(41(37,38)39)18(26)17-16(13)19(32)10-3-1-2-4-11(10)20(17)33/h1-8,27H,26H2,(H,34,35,36)(H,37,38,39)(H,28,29,30,31)

SMILES string

O=C1C2=C(C(NC3=CC=C(S(=O)(O)=O)C(NC4=NC(Cl)=NC(Cl)=N4)=C3)=CC(S(=O)(O)=O)=C2N)C(C5=C1C=CC=C5)=O

InChI key

RTLULCVBFCRQKI-UHFFFAOYSA-N

form

saline suspension

extent of labeling

2-7 mg per mL

matrix

cross-linked 4% beaded agarose

capacity

≥5 mg/mL binding capacity (human serum albumin)

storage temp.

2-8°C

Gene Information

rat ... P2rx1(25505)

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Application

Reactive Blue 4-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Blue 4-agarose has been used to express human pituitary glutaminyl cyclase (hQC) in Drosophila S2 cells.

Physical form

Suspension in 0.5 M NaCl containing preservative

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
069K5159
034K7018
071K7056
021K7077
077F9640

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C M West et al.
The Journal of biological chemistry, 271(20), 12024-12035 (1996-05-17)
A novel fucosyltransferase (cFTase) activity has been enriched over 10(6)-fold from the cytosolic compartment of Dictyostelium based on transfer of [3H]fucose from GDP-[3H]fucose to Galbeta1,3 GlcNAc beta-paranitrophenyl (paranitrophenyl-lacto-N-bioside or pNP-LNB). The activity behaved as a single component during purification over
H J Dunderdale et al.
The Journal of biological chemistry, 269(7), 5187-5194 (1994-02-18)
The ruvC gene has been cloned into the plasmid pT7-7 under the control of the T7 phi 10 promoter. Following induction with isopropyl-1-thio-beta-D-galactopyranoside, the 19-kDa RuvC protein was overexpressed to 20-30% of total cell protein. RuvC has been purified to
Paolo Natale et al.
Biochemistry, 44(17), 6424-6432 (2005-04-27)
The SecYEG complex is a membrane-embedded channel that permits the passage of precursor proteins (preproteins) across the inner membrane of Escherichia coli. SecA is a molecular motor that associates with the SecYEG pore and drives the stepwise translocation of preproteins
A A Mellati et al.
Clinical biochemistry, 26(5), 383-388 (1993-10-01)
The M2-type pyruvate kinase was purified from human meningioma by ammonium sulfate precipitation, followed by ion exchange and affinity chromatography. The specific activity of the purified enzyme was 33.4 U/mg with a yield of 6.5%. The enzyme gave a single
Matthew C T Hartman et al.
Biochemistry, 46(41), 11630-11638 (2007-09-22)
In glycosyltransferase-catalyzed reactions a new carbohydrate-carbohydrate bond is formed between a carbohydrate acceptor and the carbohydrate moiety of either a sugar nucleotide donor or lipid-linked saccharide donor. It is currently believed that most glycosyltransferase-catalyzed reactions occur via an electrophilic activation
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