R2631
Pvu II from Proteus vulgaris
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
PvuII is a DNA restriction enzyme used for molecular biology methods to cleave the recognition site 5′-CAG/CTG-3′ to generate DNA fragments with blunt termini.
Biochem/physiol Actions
Recognition sequence: 5′-CAG/CTG-3′
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.7, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C
Other Notes
Comment: This enzyme exhibits star activity when used under conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH >8.0.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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