R2756
EcoR V from Escherichia coli
buffered aqueous glycerol solution
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
EcoRV is a DNA restriction endonuclease used in molecular biology applications to cleave the recognition site 5′-GAT/ATC-3′, generating DNA fragments with blunt termini.
Biochem/physiol Actions
Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 0.1 mM PMSF, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 0.1 mM PMSF, 50% glycerol (v/v) at 4°C
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Stability of transformed antagonistic Fusarium oxysporum strains in vitro and in soil microcosms.
Migheli, Q., et al.
Molecular Ecology, 5, 641-641 (1996)
I Schildkraut et al.
Gene, 27(3), 327-329 (1984-03-01)
The cleavage site for the restriction endonuclease EcoRV has been found to be 5'-GAT/ATC-3', rather than 5'- GATAT /C-3' as reported earlier by Kholmina et al. [ Dokl . Akad . Nauk . 253 (1980) 495-497].
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Beata Podgórska et al.
Acta biochimica Polonica, 59(4), 669-672 (2012-11-07)
In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.
Diana Schenkwein et al.
Nucleic acids research, 41(5), e61-e61 (2013-01-01)
Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-specific gene insertion at desired genomic loci.
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