R3143
Ribonucleic acid, transfer, phenylalanine specific from Escherichia coli MRE 600
lyophilized powder
Synonym(s):
E. coli Phe tRNA
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About This Item
UNSPSC Code:
12352200
MDL number:
grade
Molecular Biology
form
lyophilized powder
storage temp.
−20°C
General description
Transfer RNA is isolated from E. coli MRE 600. This transfer RNA is specific for transfer of the amino acid phenylalanine.
Application
Suitable for use:
- As a tRNA standard
- As a substrate uin tRNA synthatase assays
- As a substrate in other reactions involving tRNAs
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Jiqiang Ling et al.
RNA (New York, N.Y.), 13(11), 1881-1886 (2007-09-07)
Translational quality control is monitored at several steps, including substrate selection by aminoacyl-tRNA synthetases (aaRSs), and discrimination of aminoacyl-tRNAs by elongation factor Tu (EF-Tu) and the ribosome. Phenylalanyl-tRNA synthetase (PheRS) misactivates Tyr but is able to correct the mistake using
V Serebrov et al.
Nucleic acids research, 26(11), 2723-2728 (1998-05-21)
Mature tRNAPhe from Escherichia coli and the transcript of its gene lacking modified nucleotides were compared by a variety of physical techniques. Melting experiments revealed that at a low Mg2+level the transcript was partially denatured, while the mature tRNA possessed
Noa Aharon-Hefetz et al.
eLife, 9 (2020-12-29)
Different subsets of the tRNA pool in human cells are expressed in different cellular conditions. The 'proliferation-tRNAs' are induced upon normal and cancerous cell division, while the 'differentiation-tRNAs' are active in non-dividing, differentiated cells. Here we examine the essentiality of
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