R4253
BstE II from Bacillus stearothermophilus ET
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
Recognition sequence: 5′-G/GTNACC-3′
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50% [v/v] glycerol
Other Notes
Comment: BstEII results in 10-15% activity at 37°C.
One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hour at 60 °C in the 1x reaction buffer in a total volume of 50 mL.
Supplied with 10x Reaction Buffer BstE II
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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C Kessler et al.
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