R4256
Dde I from Desulfovibrio desulfuricans strain Norway
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
DdeI is a DNA restriction enzyme used in molecular biology research that cleaves the recognition sequence 5′-C/TNAG-3′, generating 5′-cohesive termini.
Biochem/physiol Actions
Recognition sequence: 5′-C/TNAG-3′
Ligation and recutting results: After 2-10-fold Dde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65 °C incubation for 20 minutes.
Comment: Single-stranded DNA cleaves slowly.
Ligation and recutting results: After 2-10-fold Dde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65 °C incubation for 20 minutes.
Comment: Single-stranded DNA cleaves slowly.
Physical form
Solution in 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 50 mM NaCl, 1 mM DTT, 0.01% Triton X- 100 (v/v), 500μg BSA, 50% glycerol (v/v) at 4°C
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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