R4258
Apa I from Acetobacter pasteurianus
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
ApaI is a restriction endonuclease used in molecular biology applications to cleave the recognition sequence 5′-GGGCC/C-3′ , generating DNA fragments with 3′-cohesive termini.
Biochem/physiol Actions
Recognition Sequence: 5′-GGGCC/C-3′
Cutting results: a 2-10-fold Apa I overdigestion of Ad-2 DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 15 minutes.
Cutting results: a 2-10-fold Apa I overdigestion of Ad-2 DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-Mercaptoethanol, 0.02% Polydocanol (v/v), 50% glycerol (v/v) at 4°C
Other Notes
Comment: Ad-2 DNA is the substrate for the activity, cleavage, ligation, recleavage and overdigestion assays. Optimal activity is at 30 °C.
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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N Doan et al.
Journal of clinical microbiology, 38(8), 3043-3047 (2000-08-02)
Dialister pneumosintes is a nonfermentative, anaerobic, gram-negative rod that grows with small, circular, transparent, shiny, smooth colonies on blood agar. Even though D. pneumosintes has been recovered from deep periodontal pockets, little is known about the relationship between the organism
J Seurinck et al.
Nucleic acids research, 11(13), 4409-4415 (1983-07-11)
A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA
G Basile et al.
Molecular biotechnology, 35(3), 253-261 (2007-07-27)
In this work we describe the production of site-specific biotinylated human myeloid differentiation factor 88 (MyD88). A vector containing a coding sequence for a peptide derived from the carboxyl terminus of the Klebsiella pneumoniae oxalacetate decarboxylase alpha subunit was used
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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