Sma I from Serratia marcescens S_b

SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.

Recognition sequence: 5′-CCC/GGG-3′
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.

Solution in 10 mM potassium phosphate, pH 7.0, 1 mM EDTA, 300 mM NaCl, 5 mM dithiothreitol, 50% glycerol (v/v) , 0.02% polydocanol (v/v), at 4 °C

Comment: Half-life of Sma I at 37 °C is approximately 15 minutes.

Isoschizomer: Xma I. Unlike Xma I, Sma I produces blunt-ended fragments.

One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hr. at 25 °C in a total volume of 25 ml of 1x digestion buffer SA forrestriction enzymes.

Supplied with 10x Restriction Enzyme Buffer SA (B7531).