R4503
Sma I from Serratia marcescens Sb
Restriction Enzyme
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-CCC/GGG-3′
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
Application
SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Comment: Half-life of Sma I at 37 °C is approximately 15 minutes.
Linkage
Isoschizomer: Xma I. Unlike Xma I, Sma I produces blunt-ended fragments.
Unit Definition
One unit is the enzyme activity that completely cleaves 1 mg of λDNA in 1 hr. at 25 °C in a total volume of 25 ml of 1x digestion buffer SA forrestriction enzymes.
Physical form
Solution in 10 mM potassium phosphate, pH 7.0, 1 mM EDTA, 300 mM NaCl, 5 mM dithiothreitol, 50% glycerol (v/v) , 0.02% polydocanol (v/v), at 4 °C
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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