R4756
Rsa I from Rhodopseudomonas sphaeroides
Restriction Enzyme
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About This Item
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
RsaI is a restriction endonuclease that is used in molecular biology methods to cut DNA at the recognition sequence 5′-GT/AC-3′ to generate DNA fragments with blunt termini.
Biochem/physiol Actions
Recognition sequence: 5′-GT/AC-3′
Cutting results: a 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: a 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 40 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.25% polydocanol (v/v) at 4 °C
Other Notes
Comment: Rsa I cuts single-stranded DNA poorly.
Supplied with 10x Restriction Enzyme Buffer SL (B3782).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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S P Lynn et al.
Journal of bacteriology, 142(2), 380-383 (1980-05-01)
A new type II sequence-specific endonuclease, RsaI, has been identified from Rhodopseudomonas sphaeroides strain 28/5. An RsaI purification scheme that yields enzyme which is free of contaminating exonuclease and phosphatase activities after a single column fractionation has been developed. The
Characterization of nine microsatellite loci on endemic kangaroo rats Dipodomys simulans peninsularis from southern Baja California Peninsula.
Vega, A.M., et al.
Molecular Ecology Notes, 7, 127-127 (2007)
Delphine Boucher et al.
FEMS microbiology ecology, 70(1), 66-78 (2009-07-23)
This study examined the effects of temporal changes in bacterial community composition (BCC) and environmental factors on potential ectoenzymatic activities (alpha-glucosidase, beta-glucosidase, alkaline phosphatase and leucine aminopeptidase) in a lacustrine ecosystem (Sep reservoir, France). BCC was assessed by terminal restriction