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Merck
CN

R5007

Sca I from Streptomyces caespitosus

Restriction Enzyme

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About This Item

CAS Number:
UNSPSC Code:
12352204
MDL number:
EC Number:
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grade

Molecular Biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Application

ScaI is a restriction endonuclease that is used in applications in molecular biology to cleave DNA at the recognition sequence 5′-AGT/ACT-3′ to generate DNA fragments with blunt ends.

Biochem/physiol Actions

Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C

Other Notes

Comment: High concentrations of Sca I may show star activity. Use minimum enzyme concentrations for complete cleavage.
Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Meera G Nair et al.
Infection and immunity, 73(1), 385-394 (2004-12-25)
Ym1 and Fizz1 are secreted proteins that have been identified in a variety of Th2-mediated inflammatory settings. We originally found Ym1 and Fizz1 as highly expressed macrophage genes in a Brugia malayi infection model. Here, we show that their expression
K Kita et al.
Nucleic acids research, 13(19), 7015-7024 (1985-10-11)
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different
Raoudha Abdellaoui et al.
Biochemical genetics, 49(11-12), 695-703 (2011-06-18)
Genetic conservation programs in arid environments rely on molecular methods for diversity assessments. DNA-based molecular profiling will aid in conservation and protection of species from genetic erosion. Obtaining intact genomic DNA from Calligonum species, of sufficiently high-quality that is readily
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis

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