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Merck
CN

R5007

Sca I from Streptomyces caespitosus

Restriction Enzyme

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About This Item

CAS Number:
UNSPSC Code:
12352204
MDL number:
EC Number:
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grade

Molecular Biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

Application

ScaI is a restriction endonuclease that is used in applications in molecular biology to cleave DNA at the recognition sequence 5′-AGT/ACT-3′ to generate DNA fragments with blunt ends.

Biochem/physiol Actions

Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C

Other Notes

Comment: High concentrations of Sca I may show star activity. Use minimum enzyme concentrations for complete cleavage.
Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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K Kita et al.
Nucleic acids research, 13(19), 7015-7024 (1985-10-11)
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different
Meera G Nair et al.
Infection and immunity, 73(1), 385-394 (2004-12-25)
Ym1 and Fizz1 are secreted proteins that have been identified in a variety of Th2-mediated inflammatory settings. We originally found Ym1 and Fizz1 as highly expressed macrophage genes in a Brugia malayi infection model. Here, we show that their expression
Raoudha Abdellaoui et al.
Biochemical genetics, 49(11-12), 695-703 (2011-06-18)
Genetic conservation programs in arid environments rely on molecular methods for diversity assessments. DNA-based molecular profiling will aid in conservation and protection of species from genetic erosion. Obtaining intact genomic DNA from Calligonum species, of sufficiently high-quality that is readily
TALENs and ZFNs are associated with different mutation signatures.
Yongsub Kim et al.
Nature methods, 10(3), 185-185 (2013-02-12)
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential

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