R5007
Sca I from Streptomyces caespitosus
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
ScaI is a restriction endonuclease that is used in applications in molecular biology to cleave DNA at the recognition sequence 5′-AGT/ACT-3′ to generate DNA fragments with blunt ends.
Biochem/physiol Actions
Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C
Other Notes
Comment: High concentrations of Sca I may show star activity. Use minimum enzyme concentrations for complete cleavage.
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Meera G Nair et al.
Infection and immunity, 73(1), 385-394 (2004-12-25)
Ym1 and Fizz1 are secreted proteins that have been identified in a variety of Th2-mediated inflammatory settings. We originally found Ym1 and Fizz1 as highly expressed macrophage genes in a Brugia malayi infection model. Here, we show that their expression
K Kita et al.
Nucleic acids research, 13(19), 7015-7024 (1985-10-11)
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different
Raoudha Abdellaoui et al.
Biochemical genetics, 49(11-12), 695-703 (2011-06-18)
Genetic conservation programs in arid environments rely on molecular methods for diversity assessments. DNA-based molecular profiling will aid in conservation and protection of species from genetic erosion. Obtaining intact genomic DNA from Calligonum species, of sufficiently high-quality that is readily
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
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