R5268
Sac I from Streptomyces achromogenes
Restriction Enzyme
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
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Application
SacI is a restriction enzyme used in molecular biology applications to cleave DNA at the recognition site 5′-GAGCT/C-3′, generating DNA restriction fragments with 3′-cohesive ends.
Biochem/physiol Actions
Recognition sequence: 5′-GAGCT/C-3′
Cutting results: a 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate results in 100% cutting
Heat inactivation : Inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate results in 100% cutting
Heat inactivation : Inactivated at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: Sac I activity is inhibited by salt concentrations above 10 mM.
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Restriction and modification enzymes and their recognition sequences.
R J Roberts
Nucleic acids research, 11(1), r135-r167 (1983-01-11)
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Journal of agricultural and food chemistry, 57(7), 2668-2677 (2009-03-07)
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Leoni, G., et al.
J. Reprod. Fert., 119, 309-309 (2000)
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
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