R5634
Nhe I from Neisseria mucosa heidelbergensis
Restriction Enzyme
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About This Item
grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
NheI is a restriction endonuclease that is used in molecular biology to cleave DNA at the recognition site 5′-G/CTAGC-3′ to yield fragments with 5′-cohesive termini compatible with ends generated by AvrII, SpeI and XbaI.
Biochem/physiol Actions
Recognition sequence: 5′-G/CTAGC-3′
Cutting results: a 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Cutting results: a 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.8, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.2% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
Other Notes
Comment: High salt concentrations will inhibit activity of Nhe I.
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Restriction and modification enzymes and their recognition sequences.
Roberts, R.J.
Nucleic Acids Research, 13, r165-r165 (1995)
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C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
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