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R5636

Sigma-Aldrich

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

buffered aqueous solution

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
9014-25-9
MDL number:
MFCD00132200
NACRES:
NA.55

grade

for molecular biology

Quality Level

200

form

buffered aqueous solution

concentration

≥9 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
  • in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
  • as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
  • as a carrier to enhance recovery of RNA from small numbers of cells

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Regulatory Information

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Marilyn C Olson et al.
Methods in molecular biology (Clifton, N.J.), 258, 53-69 (2004-02-19)
The Invader assay is a homogeneous, isothermal, signal amplification system for the quantitative detection of nucleic acids. The assay can directly detect either DNA or RNA without target amplification or reverse transcription. It is based on the ability of Cleavase
Oliver Mühlemann et al.
Methods in molecular medicine, 131, 33-46 (2007-07-28)
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro
Quantitative Analysis of T Cell Receptor Diversity in Clinical Samples of Human Peripheral Blood
Memon SA, et al.
Journal of Immunological Methods, 375(1-2), 84?92-84?92 (2012)
Eric A Nalefski et al.
iScience, 24(9), 102996-102996 (2021-09-11)
Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound to CRISPR RNA (crRNA) recognize specific nucleic acid targets, initiating cleavage of the targets as well as non-target (trans)
Naming 'junk': human non-protein coding RNA (ncRNA) gene nomenclature
Wright MW and Bruford EA
Human Genomics, 5(2), 90-90 (2011)
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