R5636
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)
buffered aqueous solution
Synonym(s):
Transfer RNA, tRNA
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Form:
buffered aqueous solution
Quality level:
grade
Molecular Biology
Quality Level
form
buffered aqueous solution
concentration
≥9 mg/mL
foreign activity
DNase, Nickase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.
Application
Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
- in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
- as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
- as a carrier to enhance recovery of RNA from small numbers of cells
Biochem/physiol Actions
Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.
Preparation Note
tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.
Still not finding the right product?
Explore all of our products under Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Marilyn C Olson et al.
Methods in molecular biology (Clifton, N.J.), 258, 53-69 (2004-02-19)
The Invader assay is a homogeneous, isothermal, signal amplification system for the quantitative detection of nucleic acids. The assay can directly detect either DNA or RNA without target amplification or reverse transcription. It is based on the ability of Cleavase
Oliver Mühlemann et al.
Methods in molecular medicine, 131, 33-46 (2007-07-28)
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro
Naming 'junk': human non-protein coding RNA (ncRNA) gene nomenclature
Wright MW and Bruford EA
Human Genomics, 5(2), 90-90 (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R5636-5X1ML | 04061836695682 |
| R5636-1ML | 04061836695675 |